Sentence examples for mechanically isolated using from inspiring English sources
After washing with cold ES, single smooth muscle cells were mechanically isolated using a fire-polished pipette.
Cells were mechanically disrupted by beadbeating and total RNA was isolated using Trizol-chloroform extraction (Invitrogen, Carlsbad, CA).
Frozen tissue specimens were disrupted mechanically in guanidine thiocyanate buffer, and total RNA was isolated using cesium chloride gradient ultracentrifugation as described [ 42].
Cells were lysed mechanically using the FastPrep system (Bio101; Q-biogene), and RNA was isolated using the RNeasy mini kit (QIAGEN, Valencia, Calif).
The cells were mechanically disrupted using FastPrep homogenizer (MP Biomedicals) and total RNA was isolated using the RNeasy Mini Kit (QIAGEN).
The harvested cells were mechanically disrupted with mortar and pestle under liquid nitrogen and total RNA was isolated using a combination of phenol/chloroform extraction with Trizol reagent (Invitrogen, Carlsbad, CA, USA) and the RNeasy kit (Qiagen, Hilden, Germany).
Total RNA was isolated using Isogen (Wako).
Total RNAs were isolated using TRIzol (Invitrogen).
PBMCs were isolated using ficoll gradient centrifugation.
DNA was isolated using standard extraction protocols.
Total RNA was isolated using TriReagent (Molecular Research Centre).
