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Mouse liver tissues were mechanically homogenized using the PowerGen 125 Homogenizer (Fisher Scientific, Fair Lawn, NJ).
Colon tissues were then briefly mechanically homogenized using the Bullet Blender (Next Advance, Cambridge, MA) at 4°C.
This precooled buffer was added directly on ice to the frozen slices in Eppendorf tubes and the tissue was mechanically homogenized using a plastic pellet pestle.
The frozen glands were thawed and mechanically homogenized using a pestle in 120 μl of buffer (8 M urea, 4 % CHAPS and 0.002 % bromophenol blue (BFB)).
In order to recover bacteria from infected larvae, groups of zebrafish larvae were transferred individually (with 100 μl of E3) to microfuge tubes and mechanically homogenized using a micropestle (Eppendorf).
Approximately 30-50 mg of calli grown on callus induction medium was mechanically homogenized using a spatula in 250 μl of nuclei extraction buffer (solution A of the High Resolution Kit for Plant DNA, Partec, Munster, Germany).
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Precooled (−80°C) 5 mm stainless steel beads (QIAGEN) were added to the tubes with frozen cell pellets, and the samples were mechanically disrupted and homogenized using the TissueLyser system (QIAGEN).
Tissues were dissociated mechanically and further homogenized using the FastPrep-24 system according to the manufacturer's protocol (MP Biomedicals, Santa Ana, CA).
The lungs of mice were kept separately in the infecting medium (RPMI+5% FCS), minced mechanically using a mesh and homogenized using a tissue homogenizer to obtain a suspension.
The slices were homogenized using a Pro homogenizer (Oxford, CT).
Tissues were homogenized using a Kinematica homogenizer.
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