Sentence examples for mechanically disrupted using from inspiring English sources
Mycelia and conidia samples were mechanically disrupted using the TissueLyser II (Qiagen).
Cells were then mechanically disrupted using acid-washed glass beads (size 425 600 μm; Sigma Aldrich) and a FastPrep ribolyser.
Briefly, tissues were mechanically disrupted using a homogenizer (Omni International 2000) in concentrated nitric acid Suprapur (Merck, Chemical Co).
Blood clots thawed in a 37°C water bath were first mechanically disrupted using ClotSpin tubes (Gentra Systems Inc., Minneapolis, MN).
The cells were mechanically disrupted using FastPrep homogenizer (MP Biomedicals) and total RNA was isolated using the RNeasy Mini Kit (QIAGEN).
The cell pellet was then re-suspended in 25 mM Tris-HCl pH 8.0 with protease inhibitor cocktail (Roche diagnostics, Cat. No. 04906837001) and 1 mM of phenylmethylsulfonyl fluoride (PMSF) and mechanically disrupted using glass microbeads (600 μm, Sigma-Aldrich).
Total bacterial RNA was extracted from overnight cultures of GBS by using an RNeasy Mini Kit (QIAGEN, Hilden, Germany) per manufacturer's instructions, except that bacteria were mechanically disrupted by using tubes with glass beads (Lysing Matrix B, MP Biomedicals, Solon, OH, USA).
Pellets were then resuspended in 5 ml of MeOH/CHCl3 (2:1) and mechanically disrupted twice using French Press at 28,000 psi (Constant Cell Disruption System, Constant System Ltd).
The nematodes were placed in a 1.5 ml Eppendorf tube containing 20 μl of M9 medium with 1% Triton X-100 and were mechanically disrupted by using a pestle.
The brains were cut with a coronal section at the injection site, and the anterior part was mechanically disrupted and used for establishing cell cultures whereas the posterior part was formalin-fixed and used for paraffin sectioning.
The solution was mixed with 200 mg of 0.5 mm beads (TOMY SEIKO, Tokyo, Japan), and the cells were mechanically disrupted 10 times using a BeadSmash 12 (Wakenyaku, Kyoto, Japan) at 4°C, 4,000 oscillations per minute for 1 min.
