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Immediately after surgery, thymus tissue was separated from the fibrotic capsule and from visible blood-vessels, mechanically disaggregated using a scalpel and scissors followed by pipetting several times with phosphate buffered saline (PBS).
Tissue samples were stored in liquid nitrogen and mechanically disaggregated using a Micro-Dismembrator (Braun), yielding homogenous samples.
The tissue fragments were then mechanically disaggregated using Medicons™ (BD Biosciences, San Jose, CA, USA) with a 50 μm mesh size.
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Rainfall events are disaggregated using a conditional random walk on a dimensionless mass curve.
Each dried sediment sample (approximately 80 g) was crushed and disaggregated using a sodium sulfate solution.
For flow cytometry, one whole plate of d9 hEBhEB (whole well contents) were disaggregated using TrypLE and stained as above.
For flow cytometry analysis, colospheres were disaggregated using trypsin.
Once dissected from the meninges, the tissue was disaggregated using trypsin and DNaseI.
Tumors were extracted and disaggregated using 0.02% collagenase type II (Sigma-Aldrich, St . Louis MO, USA).
The ASSA2003 projections of population growth, disaggregated using the same age/sex categories as for utilisation, were used.
The tissue was treated with Trizol (Gibco, Life Technologies, Darmstadt, Germany) and disaggregated using a homogenator (Ultra Turrax T8; Ika, Staufen, Germany).
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