The chapter concludes that to enable a well-integrated system to be created, the electrical engineer needs to have both a complete operational specification and a mechanically completed functional specification.
Organs were mechanically disaggregated in complete medium (RPMI 1640 supplemented with 10% heat inactivated fetal bovine serum, 100 U/ml of penicillin, 50 µg/ml streptomycin, 1 mM L-glutamine) by gently pressing them through a 100 µm mesh using a syringe plunger.
Freshly resected breast cancer specimens provided by the FRSQ Cancer Network (as mentioned earlier) were mechanically homogenised in complete AIM-V medium with the Medimachine (Dako Cytomation) to obtain a single-cell suspension.
Cell dissociation was completed mechanically by pipetting up and down.
The dissociation of cells was completed mechanically using a glass micropipette by pipetting up and down.
One hundred and forty-seven accessions previously genotyped with 216,000 SNPs [ 35] were mechanically inoculated following a complete random 4-block design, the experiment was repeated over two years.
While in Africa Salivaria trypanosomes are transmitted both cyclically by tsetse flies and mechanically (i.e. without completing the cycle), in America only mechanical transmission by tabanids [ 2], other hematophagous fly species and even vampire bats has been observed [ 1, 3].
Whereas, GBM patient-derived neurospheres were mechanically dissociated and re-suspended in complete NeuroCult medium and seeded 2000 cells per 100 μl of complete NeuroCult medium per well as previously described (Ziegler et al, 2011).
All tests were completed on a mechanically braked cycle ergometer (Ergomedic 874E or 828E, Monark, Varberg, Sweden).
All tasks and questions were designed to be completed by nonverbal, mechanically ventilated, or restrained patients in ICU settings.
