Sentence examples for mechanically broken using from inspiring English sources

In brief, cells were mechanically broken using a 2 ml glass/glass Dounce homogenizer (Kontes) (30 strokes).

Cells were washed with ice-cold phosphate-buffered saline, harvested, resuspended in lysis buffer (10 mM NaCl, 3 mM MgCl2, 10 mM Tris-Hcl [pH 7.4]) and incubated for 30 minutes at 4°C. Cell membranes were then mechanically broken using a 2 ml glass homogenizer, and nuclei were separated from the cell debris by centrifugation.

Cells were mechanically broken using glass beads.

Pelleted E. coli cells were suspended in lysis buffer (50 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 8.0), mechanically broken by sonication using a pulse sequence of 15 s on and 10 s off and clarified by centrifugation at 4,000 rpm at 4°C for 1 h.

The ICMs were then mechanically broken down into 2 3 small clumps using a small pipette and the ICM clumps were transferred to a freshly prepared feeder layer.

It was a family of pumpkin heads that sang, but was mechanically broken.

In the top-down methods larger units are mechanically broken to get micro or nanoparticles.

In order to maximize the nutritional value of the product, the Chlorella cell wall is mechanically broken in the production process (Janczyk et al., 2007).

Cells were swollen in a hypotonic buffer, mechanically broken by pushing through a needle, and extracted with 1% Triton X-100.

These were washed and mechanically broken into small pieces followed by the liquid nitrogen treatment and ground to fine powder (size > 1 mm).

Measures used in the management of plinthic soils include mechanically breaking the hardpans and the use of organic and inorganic amendments to modify the structure and chemistry of the soils.