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These two sample mixtures were mechanically activated using high energy planetary ball mill (Ratsch) with 50 ml vial and balls of zirconia as medium in milling, individually.
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Piezo proteins are pore-forming subunits of mechanically activated channels.
For experiments on mechanically activated currents we used previously described methods [9], [10].
Activating "Use packet data" has charges.
The compound inhibited capsaicin-evoked nerve activity in afferent fibers without affecting mechanically activated activity.
More importantly, no mechanically activated currents were detected by using soma indentation (Supplementary Fig. 2).
The use of mechanically activated ceramic precursor powder allows obtaining isotropic ceramics in a single thermal treatment, in which synthesis, grain growth and sintering takes place simultaneously.
Wang, J. P., Kouznetsova, T. B. & Craig, S. L. Reactivity and mechanism of a mechanically activated anti-Woodward Hoffmann DePuy reanti-Woodward Hoffmann DePuy
Each pool was tested for activity on mechanically activated currents expressed by DRG neurons.
For analysis of the kinetic properties of mechanically activated current, traces were fit with single exponential functions using the Fitmaster software (HEKA).
Because the peptide inhibited the persistent component of mechanically activated currents with smaller effects on peak current amplitude, we used total charge transfer (from the integral of the current waveform) rather than peak amplitude as a measure of current magnitude.
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