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Injection of CbTX resulted in a significant dose-dependent antinociception as evidenced by increased mechanical withdrawal threshold and thermal withdrawal latency.
Mechanical withdrawal threshold.
Fig. 2 Changes in the mechanical withdrawal threshold of the footpad in rats.
The mechanical withdrawal threshold (MWT) test was performed to examine the paw response to mechanical stimuli as previously described [11].
Chronic (CoCl2) administration, significantly increased nociception in TF (p<0.001) and HP (p<0.001) tests and decreased the mechanical withdrawal threshold (p<0.02).
The mechanical withdrawal threshold (MWT) and the thermal withdrawal latency (TWL) tests were performed to determine mechanical and heat hypersensitivity of animals, respectively.
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All trials and measurements of mechanical withdrawal thresholds were performed on the same day.
Significant differences in the mechanical withdrawal thresholds between the two groups were observed at days 14 and 21.
Measurement of mechanical withdrawal thresholds or thermal stimulation of the foot surface has often been used, with the former indicating mechanical allodynia and the latter revealing thermal hyperalgesia.
The mechanical withdrawal thresholds showed significant differences from days 14 to 21, but some parameters showed differences between the two experimental groups from day 7.
We aimed to measure mechanical withdrawal thresholds and analyze gait patterns using the CatWalk method for the evaluation of the pain-related behavior caused by NP application.
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