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Neurons were also sensitized by this treatment showing decreased mechanical thresholds of the dural receptive sites and enlarged facial and cervical cutaneous mechanoreceptive fields as well as increased responses to noxious mechanical stimulation of paraspinal muscles [43].
However, analysis of the mechanical thresholds of C- fibers did suggest that a subpopulation of C- fibers was somewhat influenced by rapamycin (Fig. 7) and indeed the expression of raptor overlapped with non-N52 positive profiles in the sciatic nerve.
Modulation of RA currents by external Ca2+ is distinct in capsaicin-sensitive and insensitive neurons [9] and the mechanical thresholds of the two cell types are different, implying that distinct ion channels may mediate RA currents in nociceptors and non-nociceptors.
The mechanical thresholds of primary afferents were compared using Friedman's one-way anova followed by Dunn's post hoc test.
Importantly, in inflammation, the mechanical thresholds of the TRPA1-positive afferents were significantly lowered compared with normal animals, whereas the thresholds of TRPA1-negative afferents did not change.
Indeed mustard oil, a TRPA1 agonist, applied to skin lowered the mechanical thresholds of high-threshold dorsal horn neurones (Woolf et al., 1994).
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Mechanical threshold of muscle input ranged from 8.8 to 14.7 mN (mean temporal muscle 12.7 mN, neck muscles 13.6 mN).
The CFD model was employed to simulate the τMSS which will most probably exceed the mechanical threshold of the nociceptors, τth.
To observe how different treatments affect the allodynia, behavioral tests were performed 1 d before the drugs administration to provide baseline scores, and then from 4 h after SNL and for 1 w (once a day) to evaluate the gradual changes in the mechanical threshold of the rats.
This myelinated HTMR fiber had a mechanical threshold of 10 mN and neither a 5 mN mechanical stimulus nor the maximal intensity of blue light evoked a response.
The mechanical threshold of each recorded joint afferent was determined by a gradual increase of torque to the joint until the fiber started to elicit action potentials.
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