There were no differences in mechanical thresholds between nondiabetic and ob/ob diabetic mice at 8, 9, or 10 weeks of age.
Of note, there was not a significant correlation between baseline mechanical threshold and the degree of OIH observed after chronic morphine treatment (P > 0.05).
Potential differences between reports that find no change in mechanical threshold and our work are not immediately obvious but could include the site of inflammation, superficial/cutaneous or deeper tissues, and in vitro vs. in vivo preparations.
Mechanical threshold (MT) testing is widely used to measure nociceptive thresholds.
After the waiting period, participants underwent the three quantitative sensory tests for pain: heat pain threshold and tolerance, cold pain threshold and tolerance, and a second mechanical threshold.
Mechanical threshold was tested using von Frey filaments (Stoelting, Kiel, WI, USA) in a blinded manner.
Then, the change in the mechanical threshold (CMT, %) was calculated.
The flexion-elicited mechanical threshold was expressed in grams (g).
After AIA induction SNS-gp130flox/flox mice showed a reduction of mechanical threshold for withdrawal at the ipsilateral (right) paw and a slight increase of threshold at the contralateral paw (Fig. 1c, significant difference between both paws, ANOVA: F[1, 11] = 28.298; p < 0.001).
α-Spinasterol exhibited an antinociceptive effect to noxious heat, but the mechanical threshold was unaffected.
