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Mechanical threshold at the central plantar surface of the hind paw was assessed before the chimera and CFA injections (as a basal pain threshold).
On day 5, BQ-123 produced significant increase in mechanical threshold at both time points of 30 minutes and 1 hour after drug administration, compared with control group.
Through the time course of tactile sensitivity changes in animals after treatment of BQ-123, we observed similar elevated trends in mechanical threshold at both 30 minutes and 1 hour following injection, showing dose-dependent tendency.
The contracture results because −40 mV is the mechanical threshold at which Cav1.1 can interact with the ryanodine receptor to initiate intracellular calcium release (this threshold is below the threshold of the Cav1.1 central pore current of about −20 mV).
The baseline data of Fig. 1 correspond to values of our previous studies [ 3, 4, 29] [i.e., the weight-bearing is symmetric, the mechanical threshold at the noninflamed knee is slightly below 250 g (250 g is the cutoff value, average of baseline values here 234.5 g ± 20.6 g), and the mechanical threshold at the paw is around 30 g (average here 27.9 g ± 7.6 g)].
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Like mice treated with dexamethasone, bosentan-treated mice showed significantly increased mechanical thresholds at the inflamed side.
In adults, intrathecal administration of anti-IL-10 after SNI has no significant effect on mechanical thresholds at any time (McKelvey et al., 2015).
Antinociception is expressed as an increase of mechanical thresholds at the knee joint (reduction of hyperalgesia) and a faster normalization of pain-related gait disturbances.
The pressure in lbf, kgf, N, or ozf is then recorded as mechanical thresholds at the time of reaction by animal.
In this study, we have demonstrated successful use of a modified algometer to measure mechanical thresholds at five body points in dogs.
Repeated measures ANOVAs showed significant Time × Group interactions for primary mechanical hyperalgesia assessed as mechanical thresholds at the injected knee joint (F 16,141) = 1.947; P = 0.021) and for weight bearing as obtained from incapacitance testing (F 16,141) = 1.798; P = 0.042).
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