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All mechanical stretch experiments were carried out on BioFlex six well culture plates (Dunn Labortechnik GmbH) coated with 2 μg/cm human fibronectin (Sigma).
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Major concern: 1) Since Vav1-P1cKO cells are drastically different in shape I am not convinced that the mechanical stimulus applied in the calcium-imaging/membrane stretch experiments is equivalent to the control (wild-type) cells.
Reviewer #1: Major concern: 1) Since Vav1-P1cKO cells are drastically different in shape I am not convinced that the mechanical stimulus applied in the calcium-imaging/membrane stretch experiments is equivalent to the control (wild-type) cells.
We excluded neurohumorally increased mediators, mediators increased in renal tissue samples and mediators derived from in vitro experiments exposing cell cultures to mechanical stretch.
Experiments in tissues and single myocytes indicated that mechanical stretch significantly increases the production of nitric oxide (NO) and the amplitude and duration of muscle contraction.
Finally, the notion that FRET changes actually reflect changes in tension may be reinforced by experiments in which external forces are rapidly applied using mechanical stretch 5 or fluid shear flow.
Application of cyclic mechanical stretch improved contractile function, cardiomyocyte alignment along the stretch axis and gene expression of cardiomyocyte markers.
Differential proteins under mechanical stretch are also profiled, especially for those events undetectable in transcriptomic analysis.
In this study, we have shown that mechanical stretch triggers the NF-κB signaling pathway.
Fig. 2 HCA attenuates alveolar epithelial injury following 24 and 120 h of cyclic mechanical stretch.
Mechanical stretch was started at certain times (3 75 h) after seeding and continued for 24 h.
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