The overall results suggest that three dimensional (3D) scaffolds can recapitulate the mechanical stimuli required for directing hESC differentiation and that these stimuli can play a significant role in determining hESC fate.
We hypothesize that the constant mechanical stress placed on rib callus during breathing may indeed provide the appropriate mechanical stimuli required for myofibroblastic function.
Thus, global mapping of activated genes and proteins that are responsible to the specific mechanical stimuli is required to conduct from the viewpoint of omics.
The generation of effective tissue engineered bone grafts requires efficient exchange of nutrients and mechanical stimulus.
According to the diamond concept [ 33, 34], four factors are essential for new bone formation, as is required when treating a fracture: osteoconductivity, osteogenesis, osteoinductivity, and mechanical stimulus.
For secondary allodynia measurements, a von Frey hair algesiometer (Ugo-Basile, Milan, Italy) was employed to determine the force required to elicit a withdrawal response to a tactile mechanical stimulus applied to the hindpaw.
A Dynamic Plantar Aesthesiometer (Ugo Basile; Collegeville, PA, USA) was used to assess the force (in grams) required to elicit a paw withdrawal from a blunt mechanical stimulus, as previously described [ 23].
The stretch-dependent induction of Sirt1 by Egr1 is required to remove the excess of reactive oxygen species, ROS, generated by the mechanical stimulus.
The spike in intracellular calcium upon mechanical stimulus from neighboring tissues may inform the cells about the direction of the boundary, information that is required for the later polarization of MT growth [5].
