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More recently we used a similar approach to create a morphologically accurate FE model of knee joint development [30], indicating that the tempero-spatial pattern of mechanical stimuli generated in the distal femur by muscle contraction corresponds with aspects of the pattern of shape changes and with differential rates of cell proliferation in the femoral condyles.
In this work we used computational modelling to demonstrate how rigid immobilisation would affect the mechanical stimuli generated in the developing knee joint and we investigated the impact of such immobilisation on the tissues of the developing joint at morphological and molecular levels.
WBV uses high-frequency mechanical stimuli generated by a vibrating platform which are transmitted through the body [ 25].
Employing biochemical, biophysical, and mechanical stimuli generated robust hyaline articular cartilage with a tensile modulus of 2 MPa and a compressive instantaneous modulus of 650 kPa.
Understanding how mechanical stimuli generated by foetal movement impact skeletal development will augment our fundamental knowledge of tissue formation and has at least two important potential avenues for clinical application.
For example, the meshwork may function as a buffer from physical forces generated through nuclear migration or transduction of mechanical stimuli generated within the cell or from the extracellular environment.
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Given the importance of appropriate mechanical stimulation generated by embryo movement on skeletal development we postulated that mechanical stimuli must integrate with biochemical cell signalling pathways known to be essential for normal development.
The stimuli generated in developing tissues cannot currently be directly measured, however, finite element (FE) analysis, informed by the morphology of emerging tissues in the limb and direct measurement of mechanical properties of the developing interzone, can predict patterns of stimuli generated across space and time by limb flexion/extension.
Flow-derived shear forces generate mechanical stimuli concurring with biochemical signals in the modulation of leukocyte endothelial cell interactions.
Dynamic compression of 5% applied on the PLA Glass scaffold with a strain rate of 0.005 s−1 has the benefit to generate mechanical stimuli based on both solid shear strain and fluid flow shear stress on large scaffold surface area.
The in ovo situation of the chick embryo means less passive movement from external sources and therefore a greater reliance on muscle contractions to generate mechanical stimuli in the hindlimbs.
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