Sentence examples for mechanical stimulation with from inspiring English sources

It is now important to functionally test the mechanisms linking mechanical stimulation with Wnt signalling.

If we can demonstrate in an appropriate assay system that the response is direct we open the possibility of revealing the cellular mechanisms that links mechanical stimulation with gene regulation.

PLLA scaffolds with and without a calcium channel agonist, BAY K8644, were seeded with primary human bone cells or the human MG63 bone cell line and cultured for 1 3 weeks followed by mechanical stimulation with a four-point bending model.

Here, we demonstrate the long-term biocompatibility of 4.5 μm RGD-coated particles with osteoblasts up to 21 days in culture, and detail a time course of responses elicited from osteoblasts following mechanical stimulation with integrin attached magnetic particles (<2 h post attachment) and internalised particles (>48 h post attachment).

To examine the reversibility of the signal, we followed the addition of the agent and mechanical stimulation with a continuous washing procedure (lasting for 3 minutes) to remove the cytoskeletal modifiers via bath-perfusion as previously published [20].

Our findings support previous work conducted by Norvell and colleagues demonstrating that upon mechanical stimulation with unidirectional fluid flow, β-catenin is also induced to translocate to the nucleus and initiate transcription of Cox2, another osteogenic gene, in MC3T3-E1 pre-osteoblast cells [74].

There is evidence to suggest that the proximal esophagus is more sensitive to chemical and mechanical stimulation compared with the distal.

The qPCR results are reported as the mean difference between low and high cycle number and continuous and rest inserted mechanical stimulation groups with a 95% confidence interval (CI) of the difference.

However, another limitation of this study is that we did not look at tenocyte specific transcription factors such as scleraxis, which have been shown to respond to mechanical stimulation, especially with increased cyclic compression [ 30- 32] nor did we look at tenomodulin and tenascin-C [ 33], two marker genes, which are important for maintaining tenocyte phenotype [ 34, 35].

Inflammatory mediators, such as prostaglandins and pro-inflammatory cytokines (e.g., IL-6, IL-1beta, TNF) activate and sensitize nociceptors [ 25], which is manifested as increased spontaneous activity of the nociceptor and increased response to mechanical stimulation associated with joint movement [ 21, 25].

Mechanical stimulation was applied with a calibrated monofilament (North Coast Medical, San Jose, CA) with stimulation force of 260 mN on the ventral side of the right forearm or right lower leg.