Nummular headache, most frequently localized in the parietal region, seems to be associated with local increase of pain sensitivity to mechanical stimulation and trophic changes inside the painful area [3].
Paw withdrawal frequency to mechanical stimulation did not change compared to basal values and paw withdrawal latencies to thermal or cold stimulation were higher compared to the AAV5-GFP-injected mice during the observation period (Fig. 3A D).
Recently, it has also been demonstrated that the mechanical stimulation sensed by osteocytes changes significantly during osteoporosis.
Even when the indentation was close to the soma (within 30 µm), the mechanical stimulation only caused limited changes of membrane potential (<10 mV) for neurite-free neurons (Fig. 4).
Since [Ca 2+] i elevations are known to be the most common first responses to mechanical stimulation, we analyzed the changes in [Ca 2+] i in response to different loading regimes in the osteoblasts loaded with [Ca 2+] i fluorescent indicator fluo4-AM.
Fifty-eight out of the 94 patients studied exhibited spontaneous diuresis after mechanical stimulation such as a change in position on the bed, use of cold or warm bags, and stimuli from the ultrasound transducer during examination and abdominal massage.
The fluorescence signal in cells not exposed to mechanical stimulation did not demonstrate associated changes and was used as a reference for signal correction for bleaching and cantilever reflection artifacts using the protocol for data processing for systematic signal recovery that we established and described previously.
Interestingly, mechanical stimulation resulted in the same change in energy stores even when it was not associated with sleep deprivation, suggesting that the changes are caused by stress rather than sleep loss.
The degree to which the membrane potential changed following mechanical stimulation in the presence of SE5A5, however, was less than that seen in the absence of antibody, raising the possibility of a partial blocking effect.
Fluorescence data were expressed as Δ F/ Fo with the cell baseline fluorescence (Fo) representing the average of the first five images before mechanical stimulation, while ΔF represents the change in fluorescence emission.
