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This experiment was designed to test the hypothesis that ultrasound exerts a similar influence on bone as mechanical stimulation at a physiological level.
Oscillatory mechanical stimulation at relatively high frequencies (0.1 Hz) has been shown to inhibit adipogenic and promote osteogenic differentiation of mesenchymal stem cells.
Current Research and Scholarly InterestsThe auditory sensory cell, the hair cell, detects mechanical stimulation at the atomic level and conveys information regarding frequency and intensity to the brain with high fidelity.
Hydrogel construct (a) wet weights and (b) Young's moduli at day 2 and day 28 of free swelling culture (FS), or after 14 days of free swelling pre-culture followed by 14 days of intermittent biaxial mechanical stimulation at 30% compressive strain and 1 mm sliding shear amplitude (L) (1 Hz, 1 h daily).
Constructs were pre-cultured for 14 days in free swelling conditions, followed by 14 days of additional free swelling culture (FS) or intermittent biaxial mechanical stimulation at 30% compressive strain and 1 mm shear amplitude (L) (1 Hz, 1 h daily).
Viability of chondrocytes at (a) day 2 and (b) day 28 of free swelling culture, or (c) after 14 days of free swelling preculture and 14 days of intermittent biaxial mechanical stimulation at 30% compressive strain and 1 mm shear amplitude (1 Hz, 1 h daily).
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We conclude that electro-mechanical stimulation at a physiological frequency supports functional maturation in mammalian EHM.
Phasic mechanical stimulation, even at painful intensities, did not evoke a flare reaction in normal skin.
These secondary bursts were restricted to the upper most regions activated by mechanical stimulation (generally at the subdural depths of 100 200 μm, consistent with the location of laminae I-II), and occurred with a delay ranging from approximately 70 to 100 ms in relation to stimulus onset.
Evaluation of gene-based approaches to target human bone marrow aspirates in conditions of mechanical stimulation that aim at reproducing the natural joint environment may allow to develop improved treatments for articular cartilage injuries.
In our study, we found that the scaffold cultured in our bioreactor with a mechanical stimulation of stir at a speed of 300 rpm had a better reparative effect than the scaffold cultured without the stir.
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