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In addition to activation by chemical mediators, recent studies have demonstrated that mechanical forces may activate latent TGF-β via integrin-mediated cellular contractions, or mechanical shearing of blood serum.
The efficient mechanical shearing of ball milling and the hydrogen bond interactions between RGO and cellulose molecules contribute to the formation of a homogeneous RGO/cellulose hydrogel, improved thermal stability of the resultant composites, and enhanced crystallinity of the cellulose in the composites.
Two pUC118 libraries (inserts 2 3 and 6 8 kb) were generated by mechanical shearing of chromosomal DNA.
This library was constructed after mechanical shearing of genomic DNA and cloning of the resulting fragments into plasmid pCNS (pSU18-derived).
This library was constructed after mechanical shearing of genomic DNA and cloning of generated inserts into plasmid pCNS (pSU18-derived).
Mechanical shearing of the samples is also caused by the grinding action of the mixing screws inside the HS reactor.
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To simulate the effect of mechanical shearing on viscosity, 2,500 and 3,000 mg/L of the polymer solution were dissolved with formation water and simulated formation water and sheared in a Waring device at a speed of 3,000 r/min for 20 s.
Reactivation, primarily through mechanical shear, of pervasive, large-scale fractures is shown capable of causing both hydraulic and thermal short circuiting.
After secretome selection [ 40], the inserts from the resulting metasecretome ssDNA pool were PCR-amplified and processed by enzymatic and mechanical shearing to fragments of a suitable size range (600 - 800 bp) for 454 GS FLX sequencing.
For example, Aird et al. examined sources of potential bias through the course of an Illumina library preparation including mechanical shearing, ligation of adapters and the PCR enrichment step.
Plasmid libraries with small (2 3 kbp) and large (10 12 kbp) inserts were constructed in pUC-derived vectors following random mechanical shearing (nebulization) of genomic DNA [23].
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