The intact chromosomes were protected by the agarose matrix, allowing for further manipulations with minimal mechanical shearing.
Therefore, a FEA model will be developed in this paper to study the dynamic processes of cells' detachment under mechanical shearing stresses.
The nanocomposites were prepared by the in situ polymerisation method with the aid of mechanical shearing.
Moreover, when whole cells were treated by proteinase K, spinae were detached from the cells without leaving the scars on the cellular surface as observed in the case of mechanical shearing of the spinae (Supplementary information, Fig. S1, D).
The reduced cloning efficiency with longer inserts may in part result from mechanical shearing of long DNA fragments during manipulation, the lower ligation efficiency of Gibson assembly with longer DNA and the lower electroporation efficiency of larger plasmids.
Phenolic resin dispersed particle gel (PDPG) was successfully prepared from bulk gel by a mechanical shearing method.
A model is presented that uses the principles of irreversible thermodynamics to predict the reduction in consistency of a lubricating grease undergoing mechanical shearing action.
This chapter also presents other techniques, such as surface coverage, core shell structure, sonochemical, mechanical shearing, and supercritical and fluid techniques.
In-situ UV polymerization of the monomer aligned by mechanical shearing in the SA phase resulted in a macroscopically layered polymer PBIalS.
The drawback of mechanical micromanipulation is that it is low-throughput and time-consuming and can cause cellular injury from mechanical shearing during manipulation [15].
Two pUC118 libraries (inserts 2 3 and 6 8 kb) were generated by mechanical shearing of chromosomal DNA.
