Sentence examples for mechanical shaking for from inspiring English sources

Using these metrics, the most effective shaking/detachment protocol was identified as mechanical shaking for 3 h at 30 °C using a phosphate buffer with an enzyme cocktail.

An adequate amount of the homogenous powder, corresponding to 5 × 10-6 M, was accurately weighed and transferred into a calibrated flask and then dissolved in 25 ml of methanol by sonication for 10 min, followed by mechanical shaking for 10 min and lastly centrifuged for 5 min at 10,000 rpm.

The films were continuously washed with PBS under mechanical shaking for 24 h before measurements, with no observed reduction in the mean fluorescence intensity measured, showing antibodies were stably bound.

CD133-PE antibody functionalized films were continuously washed with deionized water and PBS under mechanical shaking for 24 h and then for visualisation under fluorescence microscopy (Leica, German) to evaluate the presence of bound antibodies.

About 150 mg of ethylchloroformate, 1.5 mL of methanol/pyridine solution (4/1, vol/vol) and d-2-methyl-TZCA internal standard solution (2.0 μL/mL in water) were added to the solution, and the sample was extracted with 3 mL of toluene by mechanical shaking for 20 min. The organic phase was dried to 0.1 mL under a nitrogen stream.

After addition of fresh medium, the remaining cells were allowed to recover overnight before repeating the mechanical shaking for an additional 16 h at 200 rpm to isolate OPCs.

Each solution was individually mixed with 25.0 mg of modified cellulose phases (LC, LC2, or LC3) and unmodified DEAE cellulose phase and mechanically shaken for 2 h by a mechanical shaker at 150 rpm and 25°C temperature.

Microglia, which loosely attach on top of the mixed glial cultures, were removed on day 8 by mechanical shaking at 200 rpm for 1 hr at 37°C.

As already described, for each nanofluid, the mean size value was obtained, repeating the measurements almost every day for 30 days, both for the nanofluid stored in static mode and for the same nanofluid after mechanical shaking.

The process kinetics was finally developed for comparison of the ultrasound and mechanical shaking treatments.

The physical and chemical stabilities of nanosuspensions A and B were investigated by particle analyzer and HPLC after storage for 2 months at 25 °C, 4 °C and mechanical shaking condition.