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However, mechanical sensitivity was reduced in TRPV2-deficient mice (Fig. 2c e).

Mechanical sensitivity was measured twice, before and after acupuncture treatment given to the affected hind leg in awake mice.

Mechanical sensitivity was measured using the von Frey filament touch test 3 days after CFA injection (Fig. 1A).

In the experiments testing local caffeine administration (Fig. 3A), mechanical sensitivity was first measured twice, before and after the acupuncture treatment, without caffeine on the first day.

Baseline mechanical sensitivity was measured using von Frey filament, then pain was induced by administration of complete Freund's adjuvant (CFA) in the ankle joint.

Mechanical sensitivity was measured using the Semmes-Weinstein von Frey Aesthesiometer (Stoelting Co, Wood Dale, IL) touch test, as previously described with slight modification1.

The hallmark of peripheral sensitization is represented by increased thermal sensitivity, most likely supported by ERK activation in the cell body of nociceptors followed by synthesis of TRPV1 receptors and their transport to the axon terminals in the inflamed cutaneous tissue, whereas increased mechanical sensitivity is mainly due to central sensitization of spinal cord neurons [ 65].

This increased mechanical sensitivity is mediated by capsaicin-insensitive A-fibers and reflects central sensitization within the central nervous system not peripheral sensitization (Magerl et al., 2001).

The globally optimized ring thickness distribution providing the maximal mechanical sensitivity is obtained and detailedly discussed.

It remains unclear whether an increased intrafusal mechanical sensitivity is related to the vibration-induced performance improvement.

Mechanical sensitivity is explained by size: the smallest link(s) are disproportionately affected by length changes and most strongly influence mechanical output.