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Mechanical paw withdrawal threshold (PWT) was used to assess pain behavior.
The pain response was examined behaviorally with mechanical paw withdrawal and thermal paw withdrawal method.
To assess pain perception, a mechanical paw withdrawal threshold (PWT) test was performed prior to BCP surgery and at d 3, 7, and 14.
Nociceptive thresholds were assessed with the mechanical paw withdrawal threshold test each day after the conditioning procedure.
The administration of intrathecal nicotinic acetylcholine receptor agonists reduced mechanical paw pressure thresholds with a potency of epibatidine = A-85380 >> nicotine > choline in the normal rat.
We found that LXA4 increased Basso, Beattie and Bresnahan (BBB) scores, increased mechanical paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) to a radiant heat, reduced the lesion volume, decreased Bax mRNA expression and increased Bcl-2 expression after SCI.
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Cytokine concentrations in the serum were assessed with a 10-plex cytokine panel.Weight bearing was not affected by sham or MMT surgery; however, the MMT group had decreased mechanical paw-withdrawal thresholds in the operated limb relative to the contralateral limb (P = 0.017).
Weight bearing was not affected by sham or MMT surgery; however, the MMT group had decreased mechanical paw-withdrawal thresholds in the operated limb relative to the contralateral limb (P = 0.017).
Evaluation were performed on paws using mechanical noxious (Paw pressure test) or non-noxious (Electronic Von Frey test) stimuli, and a decrease of articular pain was directly measured on the damaged joint (PAM test).
Mechanical hyperalgesia (paw pressure) was assessed by applying increasing pressure stimulus on the hind paw's dorsal surface using an analgesymeter (no. 7200; Ugo Basile) and recording the degree of pressure at which paw withdrawal was induced (22).
For example, locally administered oxidized ATP, an irreversible inhibitor of P2X7 receptors, had an antihyperalgesic effect on complete Freund's adjuvant-induced mechanical hyperalgesia (paw pressure test) [ 68].
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