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Mechanical inoculation experiments onto indicator plants showed that Physalis floridana and Nicotiana glutinosa 'PRI' were hosts for the spherical virus, but not for PepMV.
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This last evolutionary phenomenon is very likely to have taken place in our evolution experiment, as the experimental procedure of serial transfers by mechanical inoculation imposed narrow bottlenecks.
Here, the mechanical inoculation was used TuMV YC5-infected N. benthamiana tissue as inoculuns.
Here, the mechanical inoculation was used 4 local lesions of C. quinoa tissue as inoculums.
At the end of the inoculation experiments, 14C-mass balances were calculated.
All inoculation experiments used an overnight accumulation culture grown to stationary phase in advance.
The mechanical inoculation of PVX-HER in N. benthamiana was inconsistent with the symptoms in the indicator plants.
Three treatments per labeling experiment were established, with each treatment prepared as described for our inoculation experiments.
Inoculum and mechanical inoculation procedures were as described previously [62].
Infection experiments were run as incurred matrices in 2 consecutive stages, described below as inoculation experiments I and II.
Inoculation experiments were conducted with onion epidermis.
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