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A similar protocol was adopted in testing mechanical hyperalgesia on the abdomen.

All doses of tumor cells tested induced significant mechanical hyperalgesia on day 8, which remained on days 10 and 12.

Mechanical hyperalgesia, on the other hand, seems to require the induction of changes in gene expression that eventually lead to central sensitization, which maintains mechanical hyperalgesia (Lewin & Mendell, 1993; Lewin et al., 1994).

To investigate the underlying mechanism by which JTE-052 ameliorates the decrease in spontaneous locomotor activity in AIA rats, the effect of JTE-052 on the mechanical hyperalgesia of AIA rats when administered after the onset of arthritis was evaluated.

However, the authors did not definitively address the question of whether NGF-induced mechanical hyperalgesia depends on sensory neuron-derived BDNF.

At two time points before AIA induction (baseline) and on days 1, 3, 7, 14, and 21 of AIA, secondary mechanical hyperalgesia was determined on ipsi- and contralateral hindpaws by using a dynamic plantar aesthesiometer (Ugo Basile, Comerio, Italy).

Assessment started with clinical testing for mechanical hyperalgesia, mechanic dynamic allodynia as well as hypoaesthesia.

Mechanical hyperalgesia was assessed on day 22 by measuring the paw withdrawal threshold (PWT) as previously described [ 14] using a pressure analgesymeter (Unicom, Chiba, Japan).

This finding could well explain heterosynaptic mechanisms underlying mechanical hyperalgesia.

In addition, mechanical thresholds of the subjects were measured to determine whether cupping has an effect on mechanical hyperalgesia in patients with chronic neck pain.

In contrast, little effect was observed on mechanical hyperalgesia when etanercept treatment was commenced 3 days after injection of CFA (Fig. 1c).