Thea Sharrock's production treats the play as a character study rather than a mechanical gag-fest and yields two glowing performances.
In "Le Brasier Ardent," however, Mosjoukine often suggests a Slavic Buster Keaton, with his deadpan performance, fascination with multiple personalities (Mosjoukine plays no fewer than 11 roles here), and skill in constructing complex, mechanical sight gags.
The positive effect of mechanical stimulation on GAG expression during the differentiation of MSCs into chondrocytes was observed in our previous study too (Karkhaneh et al. 2014).
In this study, a multiscale mathematical model was developed to quantify the effect of mechanical loading on GAG synthesis.
In contrast, 0.5 ng/mL IL-1α preconditioning provided even greater protection against degradation, with mechanical stiffness and GAG content higher than positive control samples (p < 0.05), and more importantly, similar to negative control constructs (p > 0.05, Fig. 2b).
At day 28, control constructs continued to elaborate matrix and increase stiffness, while those constructs exposed to low doses of IL-1α showed significantly lower mechanical stiffness and GAG composition, demonstrating a concentration-dependent degrading action of the cytokine.
IL-1ra released from microspheres attenuated the degradative effects of IL-1β as defined by mechanical properties, glycosaminoglycans (GAG) content, nitric oxide production and mRNA expression of inflammatory mediators for 7 days, and continued to limit functional degradation for up to 20 days.
Although the initial reduction in mechanical properties of GAG-depleted specimens after stress relaxation was greater, the extent of recovery was similar to that seen in intact specimens.
Compressive mechanical properties and glycosaminoglycan (GAG) content of METS and individual constructs increased significantly over time.
In contrast, constructs preconditioned with 0.5 ng/mL IL-1α continued to exhibit partial protection from catabolic degradation, retaining 76%% of their mechanical stiffness and comparable GAG content (p = 0.98) compared to control samples.
In this study, we examined the effects of varying collagen concentration and crosslink density on the biological, structural and mechanical properties of collagen-GAG scaffolds for bone tissue engineering.
