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Application of mechanical force started at day 7.
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The half of rats were subjected to external mechanical force via treadmill exercise, the exercise started at day 7 postoperatively for 30 consecutive days and at a constant speed 8 m·min−1 for 45 min·day−1, while the rest served as a control.
Interestingly, at the early stage (the first 10 days after loading started) of bone healing, the defect area of the mechanical force group is not significantly different from that of the control group, revealing that mechanical force did not play the role of promoting bone formation.
Such methods require specialized equipment and a mechanical force to provide the deformation mechanism.
Ng, Besser et al. found that when an epithelial cell in a cluster started to divide to form two new cells, the cell exerted less mechanical force on its neighboring cells.
At day 7 when loading started, the difference in the CSC filled defect area was not significant between two groups: 9.28±0.36 mm in the mechanical force group and 9.34±0.74 mm in the control, respectively (P=0.859).
The source of this extrinsic mechanical force remains unknown.
We load DNA into the confinement from the top, without the need of any mechanical force, overcoming complex mechanical loading.
Baldus, I. B. & Grater, F. Mechanical force can fine-tune redox potentials of disulfide bonds.
This property could be problematic for mechanosensing, where mechanical force should be constantly monitored over time.
Albeit far less studied, mechanical force is yet another way to orthogonally catalyse chemical reactions.
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