Sentence examples for mechanical dissociation protocol from inspiring English sources
Mechanical dissociation protocol of tumour tissue has been previously described (Weiswald et al, 2009).
Indeed, colospheres are formed after a simple mechanical dissociation protocol, preserving small pieces in cultured tumour bulk, whereas colon cancer spheres are generated after an enzymatic treatment leading to a single-cell suspension.
Because a mZBF (modified zinc-based fixative) was previously shown to preserve nucleic acids better than the standard zinc fixation methods (Lykidis et al., 2007), we evaluated intracellular, extracellular and nuclear proteins, as well as post-translational modifications to histone tails with mZBF after a mechanical dissociation protocol.
To determine whether the property to form such colospheres from patient tumours was a feature restricted to cancer tissue, the same mechanical dissociation protocol was carried out in parallel with non-tumoral colon tissue counterparts from 120 of these 203 tumour tissues.
We modified a mechanical dissociation protocol (Chassevent et al., 1984) for creating single-cell suspensions of CNS tissues by pushing the tissue through a pre-moistened 100 μm filter with a syringe plunger and flushing the filter with ice-cold HBSS supplemented with 0.01 mg/ml DNase and 0.1 mM EDTA.
Various protocols using mechanical dissociation for the preparation of testicular cell suspensions have been described [ 7].
After mechanical dissociation using sterile micropipette tips, dissociated neurons were resuspended in DMEM and centrifuged.
After mechanical dissociation using sterile micropipette tips, dissociated neurons were resuspended in DMEM medium and centrifuged.
After mechanical dissociation using sterile micropipette tips, dissociated neurons were resuspended in Dulbecco's; modified eagle medium and centrifuged.
Mammospheres, appearing as spheres of floating viable cells, were collected by centrifugation and dissociated with 0.05% trypsin followed by mechanical dissociation with a Pasteur pipette.
We harvested purified primary BC cells first by mechanical dissociation and then by using the Cancer Cell Isolation Kit (Panomics Solutions, Affymetrix, Santa Clara, CA, USA) as per manufacturer's protocol.
