Sentence examples for mechanical dissociation of from inspiring English sources

Primary dissociated breast tumour (DT) cultures were isolated by density gradient centrifugation after mechanical dissociation of primary triple negative breast cancers and propagated as described (Bayliss et al, 2007).

Mechanical dissociation of hypothalamic tissue was done by gentle pipetting up and down with a sterile glass Pasteur pipette.

Briefly, cells were isolated by mechanical dissociation of the tissue on 200 µm pore nylon gauze and then passed through a nylon mesh with 60 µm pores.

During the final wash, mechanical dissociation of the cells into a single-cell suspension was achieved by pipetting up and down several times.

EBs were formed by mechanical dissociation of cell clusters and plating into low-adherence dishes in hESC medium without bFGF2 for 7 days.

Mechanical dissociation of the mixed glial cells was performed using flamed Pasteur pipets and the cells were plated at a density of 200000 cells/cm2 in poly-D-lysine and laminin-coated plates.

Single-cell suspensions from spinal cords were obtained via mechanical dissociation on a cell strainer.

Various protocols using mechanical dissociation for the preparation of testicular cell suspensions have been described [ 7].

Neurosphere cultures were then passaged every 11 days, by mechanical dissociation, to give a concentration of 50 000 cells per ml in fresh medium, in non-coated T25 or T75 flasks.

After each round of mechanical dissociation, pieces of tissues were decanted and supernatant was replaced with fresh BSS solution.

Mechanical dissociation protocol of tumour tissue has been previously described (Weiswald et al, 2009).