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These cells often displayed extensive processes in-vitro and might therefore be particularly susceptible to damage during mechanical dissociation, especially given that the DG neurospheres had a tighter structure and were thus more difficult to dissociate compared to vSVZ neurospheres.
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After mechanical dissociation using sterile micropipette tips, dissociated neurons were resuspended in DMEM and centrifuged.
After mechanical dissociation using sterile micropipette tips, dissociated neurons were resuspended in DMEM medium and centrifuged.
After mechanical dissociation using sterile micropipette tips, dissociated neurons were resuspended in Dulbecco's; modified eagle medium and centrifuged.
Mammospheres, appearing as spheres of floating viable cells, were collected by centrifugation and dissociated with 0.05% trypsin followed by mechanical dissociation with a Pasteur pipette.
For mechanical dissociation, small tissue pieces were loaded into Medicons and then dissociated according to the manufacturer's instructions.
Tumour biopsies were dissociated into single cell suspensions with collagenase digestion and/or mechanical dissociation.
Day 14 EBs were dissociated by treatment with Accumax™ Solution (Sigma) at 37 °C followed by mechanical dissociation (as described previously27).
Single-cell suspensions from spinal cords were obtained via mechanical dissociation on a cell strainer.
Colonies were broken into uniform-size clumps (see Fig. 5B) by mechanical dissociation after dispase treatment and then cryopreserved.
While oxidation of the pyrrole monomer causes growth of the film, brief subsequent reduction allowed mechanical dissociation from the electrode mold, creating a stand-alone, conductive PPy tube.
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