This might introduce mechanical disruptions to the movements of cells lining the fissure that do not occur when RPE generation is disrupted in the central retina.
Recent studies suggest that, after lipoaspiration, more than 30%% of harvested mature adipocytes and up to 50%% of AT progenitors are lost due to both mechanical disruption and to localization of progenitor cells in perivascular regions of mid-size and large vessels that are generally spared during lipoaspiration [ 5, 6].
Tissue homogenates were prepared by mechanical disruption and used to determine viral titers by plaque formation assay performed in MDCK-L cells in the presence of trypsin [34].
A volume of 600 µl buffer AL (QIAamp DNA mini kit, Qiagen) and glass beads (∼20%, diameter 150 212 microns, Sigma) were added to each sample and subjected to mechanical disruption in a FastPrep (setting of 6.5 ms−2 for 45 seconds).
This treatment is believed to cause a mechanical disruption of tumor vasculature leading to enhanced cellular damage when combined with radiation (Al-Mahrouki et al., 2012; Czarnota et al., 2012; Tran et al., 2012; Kim et al., 2013).
Titanium alloys in orthopaedic implants are susceptible to mechanical disruption of the passive film (fretting corrosion).
Resistance to mechanical disruption hinges primarily on homeostasis of the dermal collagen fibril architecture, which is regulated, at least in part, by members of the small leucine-rich proteoglycan (SLRP) family.
The swollen pancreas was excised and incubated at 37°C for 15 min. The digestion of pancreas stopped by the addition of 20 mL cold HBSS followed by shaking for 1 min to mechanical disruption.
Whole intestinal tissue was subjected to mechanical disruption under liquid nitrogen by using a mortar and pestle.
Finally, the lysate was subjected to mechanical disruption with a bead beater (BioSpec Products, Inc., Bartlesville, OK), on the Homogenize setting for 3 minutes.
