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We analyzed the DC populations liberated from the normal MLN and from the MLN undergoing sub-lethal influenza infection by two methods, mechanical disruption on or enzymatic digestion.
Pancreas, pancreatic (pLN) and a mixture of inguinal, axillary, cervical, mandibular, popliteal and mesenteric LN were excised and processed separately to obtain single cell suspensions by mechanical disruption on Nitex filters in PBS containing 5% FCS at 4°C.
Description of the biomass feedstock handling, dilute acid pretreatment in each of the different reactors, compositional analysis, and enzymatic digestibility are described in detail in the companion study, 'Effect of mechanical disruption on the effectiveness of three reactors used for dilute acid pretreatment of corn stover Part 1: chemical and physical substrate analysis' in this issue.
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Traditional tumescent, small-cannula, suction-assisted liposuction (SAL) is based on mechanical disruption of adipose tissue by a suction cannula moved manually through the subcutaneous space aspirating small fat clusters of adipose tissue through the openings in the cannula [ 4, 9, 11, 12, 21].
Here, we wanted to show a potential effect on a forming biofilm representing the in vivo situation after mechanical disruption of a biofilm and on a 42 h-old biofilm.
The effects of the mechanical disruption of the thin actin network of statocytes on gravisensitivity have been studied on lentil roots.
However, the multinucleate cells isolated from late larval stage worms are unlikely to be derived from the large Hyp7 syncytium, which would be broken apart on dissolution of the cuticle and mechanical disruption of cell contacts.
Inhibition of cytoskeletal remodeling had little impact on the cell injury, indicating that mechanical disruption of the cytoskeleton is less important than plasma membrane disruption [ 44, 45].
While it is premature to translate the in vitro data reported here to treatment recommendations, it is possible that treatment that disrupt mechanical tension and deplete disease-associated collagen may have beneficial effects on disease progression and recurrence beyond mechanical disruption of the disease cord alone.
Total RNA was isolated using the RiboPure-Yeast Kit (Ambion) using mechanical disruption of fungal cells and purification of RNA from the lysate on an RNA-binding column.
Titanium alloys in orthopaedic implants are susceptible to mechanical disruption of the passive film (fretting corrosion).
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