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However, with high-quality crust cover across this study site, recovery from small-scale disturbances such as individual fires, presence of invasive species, or mechanical disruption from mammals may have proceeded more rapidly than if a larger area was severely degraded; recovery rates of soil lichens are positively related to nearby undisturbed lichen cover (Lalley and Viles 2008).
Omsland and Heinzen (Bochner et al., 2008) have recently reported success in using information from PM assays on Coxiella burnetii that were recovered after mechanical disruption from host mammalian cells.
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The steam pretreated substrates underwent significant mechanical disruption, resulting from the quick discharge (explosion) of the biomass substrate, resulting in significant fiber shortening.
These changes protect cellular sheets from mechanical disruption and dramatically restrict the freedom of cells to roam within the monolayer.
The EM morphology suggests that: (1) the free ELS in the alveolar air spaces could result from mechanical disruption of the septum-lining basement membrane by the rigid ELS, whose ends appear sharp and broken; and (2) the structural changes observed, evidently associated with formation of the ELS aggregates, constitute the major pathological changes in that lung tissue.
These results support the notion that both water- and detergent-soluble Aβ are important in AD and are not simply released from plaques by mechanical disruption.
Amongst the various cross-linking enzymes which protect proteins from proteolysis and mechanical disruption is transglutaminase 2 (TG2) (reviewed in [3,4]).
Neutrophils were extracted from spleen by mechanical disruption of the tissue followed by positive selection with Ly6G microbeads (Miltenyi Biotec) and the AutoMACS system (Miltenyi Biotec).
RNA was extracted from scaffolds after mechanical disruption with grinding pestles, using a standard chloroform isopropanol extraction method following manufacturer's instructions (Invitrogen).
Cells were obtained from tissue by mechanical disruption and enzymatic digestion with DNAse I (80 mg/ml), hyaluronidase (2000 U/ml) and collagenase (40 mg/ml) (Sigma-Aldrich, St . Louis MO).
All of the aforementioned single cell WGA reactions were performed either on non adhering cells (e.g. sperm, blood cells) or on cells that were obtained from tissues by mechanical disruption and/or enzymatic digestion of the tissue and re-suspension.
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