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These changes protect cellular sheets from mechanical disruption and dramatically restrict the freedom of cells to roam within the monolayer.
Splenocytes were prepared by mechanical disruption and isolation over a Ficoll gradient.
Single cell suspensions of whole spleens were obtained by gentle mechanical disruption and ACK lysis (Gibco).
A single cell suspension was prepared by mechanical disruption and followed by a passage through a sterile stainless steel screen.
Single cell suspensions were obtained by mechanical disruption and passage through a 70-µm cell strainer (BD Biosciences, San Jose, CA).
Single-cell suspensions were prepared from lymph nodes by mechanical disruption and cells were stained with antibodies available commercially (eBioscience or BD Biosciences).
Similar(38)
The effect of low-temperature and high-temperature blanching, as well as the effect of two types of mechanical disruption, blending and high-pressure homogenisation, on the flow properties of carrot purée was explored.
After one month, organ cell suspensions were prepared by mechanical disruption, stained, and subsequently analyzed by flow cytometry.
Traditionally, these measurements have been performed using various configurations of electrodes, but these can present numerous challenges such as mechanical disruption of cells and tissues and an inability to measure from many sites simultaneously with high spatial resolution (Salzberg, 1989).
Spinal cord injury consists in part of mechanical disruption of neuronal and vascular tissue and much of it could be irreversible.
For example, the lesion in Figure 7 might have arisen from a high-pressure wave transmitted through the vessels supplying the piriform cortex and the lateral olfactory tract resulting in compression and mechanical disruption of the neighboring tissue and causing a tear through which the lateral olfactory tract was avulsed.
More suggestions(14)
technical disruption and
mechanical failure and
mechanical obesity and
mechanical chemical and
mechanical claw and
mechanical ventilation and
mechanical plot and
mechanical activity and
mechanical disturbance and
mechanical shock and
mechanical obstruction and
mechanical instability and
mechanical damage and
mechanical dissociation and
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