Sentence examples for mechanical disaggregation with from inspiring English sources

Cells were prepared for injection by trypsinization, sometimes in combination with collagenase treatment (1 mg/ml collagenase type IV, 15'), or by mechanical disaggregation with a Pasteur pipette.

All the mice were killed under ether anaesthesia at 28 days after implantation for evaluation of the arising tumours' malignancy and autopsy; simultaneously we removed the subcutaneously growing tumours aseptically to assess whether the arising tumours had acquired malignant phenotype, and used them for establishing individual culture cell lines after mechanical disaggregation with scissors.

Dental follicle fragments obtained after mechanical disaggregation were divided into two parts, with one part maintained in culture, while another part underwent cryopreservation.

Briefly, primary glioblastoma cells were isolated by mechanical disaggregation from surgical specimens and cultured in DMEM supplemented with 10% fetal calf serum (Biochrom), 1 mM glutamine (Biochrom), 1% penicillin/streptavidin (Biochrom) and 25 mM HEPES (Biochrom).

Briefly, IEL and epithelial cells were liberated from the mucosal specimens by mechanical disaggregation.

The histological and immunohistochemical findings in each case were reviewed.FCI detected a clonal B-cell population in 88.9% of cases processed by the manual tissue disaggregation method, 66.7% of cases processed by a combination of the manual and mechanical disaggregation methods, and in 62.7% of cases processed solely by the mechanical tissue disaggregation method (P < 0.01 Fisher exact).

It is based on mechanical disaggregation of the tissue followed by enzymatic digestion and cell purification by sequential sieving.

Spleen cell suspensions were obtained from every mouse by mechanical disaggregation and lysis of red blood cells.

Tumor and normal tissues were processed by using the same methodology, based on mechanical disaggregation of tissue followed by enzymatic digestion and cell purification by sequential sieving.

A single cell suspension was obtained by mechanical disaggregation in 0.25% trypsin for 5 min, followed by digestion in 200 µg/mL DNase1 for 1 min, and passage through a 40-µm cell strainer.

Two combined mechanical and enzymatic disaggregation techniques and a simple mechanical disaggregation procedure were compared.