Sentence examples for mechanical disaggregation of from inspiring English sources
It is based on mechanical disaggregation of the tissue followed by enzymatic digestion and cell purification by sequential sieving.
Tumor and normal tissues were processed by using the same methodology, based on mechanical disaggregation of tissue followed by enzymatic digestion and cell purification by sequential sieving.
Most other published protocols have relied on enzymatic dispersal of tumour fragments following mechanical disaggregation of tumour fragments.
Single cell suspensions from 14 human cancers were prepared by 23-gauge needle aspiration or mechanical disaggregation of surgical specimens, stained using mBCl and examined by flow cytometry.
We decided to undergo a mechanical disaggregation of tumour samples due to the softness of brain samples, avoiding enzymatic stress that could change the cell surface and even their gene expression.
After mechanical disaggregation of the tumour tissue into 1 2 mm pieces, the minced tissue was enzymatically digested with 2 mg/ml collagenase B (Roche Diagnostics, Mannheim, Germany) for approximately 20 hours under constant rotation at 37°C.
Spleen cell suspensions were obtained from every mouse by mechanical disaggregation and lysis of red blood cells.
The present investigation, using various rosetting assays of cell suspensions prepared by mechanical disaggregation or collagenase digestion, demonstrated lymphoreticular cells in human normal brain (cerebral cortex and cerebellum) and in malignant brain tumours.
Briefly, IEL and epithelial cells were liberated from the mucosal specimens by mechanical disaggregation.
Dental follicle fragments obtained after mechanical disaggregation were divided into two parts, with one part maintained in culture, while another part underwent cryopreservation.
Cells were prepared for injection by trypsinization, sometimes in combination with collagenase treatment (1 mg/ml collagenase type IV, 15'), or by mechanical disaggregation with a Pasteur pipette.
