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Briefly, IEL and epithelial cells were liberated from the mucosal specimens by mechanical disaggregation.
Dental follicle fragments obtained after mechanical disaggregation were divided into two parts, with one part maintained in culture, while another part underwent cryopreservation.
It is based on mechanical disaggregation of the tissue followed by enzymatic digestion and cell purification by sequential sieving.
Spleen cell suspensions were obtained from every mouse by mechanical disaggregation and lysis of red blood cells.
Cells were prepared for injection by trypsinization, sometimes in combination with collagenase treatment (1 mg/ml collagenase type IV, 15'), or by mechanical disaggregation with a Pasteur pipette.
Tumor and normal tissues were processed by using the same methodology, based on mechanical disaggregation of tissue followed by enzymatic digestion and cell purification by sequential sieving.
Similar(39)
We evaluated colony formation in soft agar by cells obtained after mechanical and/or enzymatic disaggregation of 455 malignant human tumours.
Preparation was performed by mechanical dissection using an automated disaggregation system (Medimachine; Becton Dickinson, Heidelberg, Germany) as described elsewhere in detail (Maaser et al, 2001).
Homogeneous distribution of the filler in the polymer matrix as well as complete dispersion (disaggregation) of the particles are prerequisites for studying the influence of other parameters on the mechanical properties of composites.
"Nothing mechanical.
Mechanical terriers?
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