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Compounds that disrupt the BRC-RAD51 interaction were identified by measuring yeast viability.
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The increased ethanol yield can be clearly attributed to the increased yeast viability measured by counting the colonies on yeast extract peptone dextrose (YPD) plates.
The yeast viability was measured as number of colony-forming unit (CFU/mL) after sampling a 24-h growth culture, serial dilutions up to 10−8 in sterile physiological water, spread of 100 μL of each dilution on YMD agar plates followed by incubation 48 h at 30°C, and counting colonies on plates containing between 30 and 300 colonies.
This region is found to be required for Prp19 function and yeast viability.
Previous studies have demonstrated the efficacy of nutrient supplementation at this point in time on yeast viability and vitality.
During the tests, controls for yeast viability were also performed.
BiP contains a conserved cysteine that is dispensable for yeast viability.
The semi-quantitative ('spotting') yeast viability assay was performed as previously reported.
This analysis also reveals that the NQ mutant promotes yeast viability without restoring Cdc6 ATPase activity.
Lactic acid bacteria did not show this detrimental effect on yeast viability.
Above 40 °C, yeast viability decreases considerably, which affects ethanol production (Suryawati et al. 2008).
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