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Scanning protocol and CAC measuring were done according to a previously published protocol using the modified Agatston method [ 15].
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In other words, within just two minutes, measuring was done.
GPC and malt quality of all samples were measured, three measurements were done for each sample.
Measuring was done in a real apartment and not in a laboratory.
Study limitations include that blood chemistry data were only available at baseline and no subsequent measuring was done during or at the end of the trial.
Cross-sectional analyses of MRI measures were done using baseline measurements.
Most of the measures were done in a shear area at embedding section with strain gauges because the deformations were of high values.
All measures were done in a noise-reduced chamber prior to the experiment.
Quadriceps strength, maximal oxygen consumption, and body composition measures were done as described elsewhere [21], [22].
Target validation and protein measures were done for selected genes to further confirm functional relevance.
The measures were done in duplicate.
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