Sentence examples for measuring transfection efficiency from inspiring English sources

A FAM-labeled negative control LNA oligonucleotide (Ambion, # AM 17012) was transfected in the same conditions as those used for the unlabeled LNA molecules, and used for measuring transfection efficiency by fluorescence microscopy at 48 hours after transfection.

To measure transfection efficiency, HBE and HELMF cells were transfected with a CMV-driven GFP reporter gene, and the number of GFP fluorescing cells was expressed as a proportion of DRAQ-5-stained nuclei.

Cells were co-transfected with pEGFP-C3 so as to measure transfection efficiency using GFP expression.

AIP1-luciferase reporter construct plasmid DNA was co-transfected with pRL-TK Renilla luciferase expressing vector to measure transfection efficiency (Promega).

In addition to the 5 µg plasmid DNA, 100 ng of GFP containing plasmid was added in order to measure transfection efficiency.

Again, we measured transfection efficiency in each experiment (Fig 2D).

Alexa 568 labeled negative control siRNA (Qiagen) was used to measure transfection efficiency.

The respective fitted value to each measured transfection efficiency was also deduced from the established model.

To measure transfection efficiency, cells were electroporated with EGFP-pCIpA102 mRNA [ 14] encoding the enhanced green fluorescence protein and analysed by flow cytometry (FACSCalibur, Becton Dickinson).

To measure transfection efficiency, the cells were fixed in 4% PFA for 30 min, washed with PBS and analyzed using Accuri C6 flow cytometer.

A FAM-labeled control premiR (Applied Biosystems) or a Block-it fluorescent oligonucleotide with no human homologous sequences (Invitrogen) was used as a control and to measure transfection efficiency in premiR and siRNA experiments, respectively.