A kit based on the Bradford method (Nanjing Jiancheng Technology Co., Ltd., Nanjing, China) was used to measure tissue protein content.
However, even in severe cirrhosis sinusoidal fenestra are still present [ 33] and the liver lymph protein/plasma ratio (which is a measure of tissue protein) is about 0.6, much greater than the corresponding ascites protein ratio of 0.17 [ 13].
cGMP was measured by radioimmunoassay and normalized to the total tissue protein measured using a bicinchoninic acid protein assay kit (Perbio Science, Northumberland, UK).
The membrane protein:total protein ratio was evaluated from the measures of total tissue protein required for the ChAT assay and of membrane protein required for the ligand binding determinations.
Tissue protein expression, measured semi-quantitatively, presented a higher value in the biopsies with a pathological diagnosis of IF/TA.
Calculated values of 79±± 9 and 83 ± 4 pg/mg tissue protein were measured for MCP-1 (CCL2) in WT and MC1R mutant mice.
Cardiac tissue protein carbonyl was measured using OxiSelect protein carbonyl ELISA kit (Cell Biolabs, San Diego, CA, USA).
Tissue cytokine mRNA concentrations were measured by real-time PCR and tissue protein concentrations by cytometric bead array.
Tissue protein content (in milligram protein per sample) was assessed by a Bio-Rad Protein Assay (Bio-Rad, Hercules, CA, USA), and absorbance was measured with an EL808 microplate reader (BioTek, Winooski, VT, USA).
Tissue protein was prepared as described [48].
