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Thrombus size was calculated at 1-minute intervals after injury by measuring thrombus surface area (TSA) using ImageJ software.
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The methods to measure thrombus formation was performed as described previously [ 11, 12].
To measure thrombus formation along the whole capillary, lysis buffer was passed through each capillary and protein concentration of the lysates measured as an indication of platelet number.
Apart from aiding in the formation of thrombus, surface bound protein has been shown to increase the level of bacterial adhesion, increasing the chance of biofilm formation and infection30.
The experimental setup models in vivo conditions when neutrophils start to adhere to platelet aggregates on a thrombus surface.
IRTME measures the surface temperature of pigs.
Clot burden was defined either on thin-slice non-enhanced CT, CT-angiography, or recently on T2* gradient-echo images measuring either absolute thrombus length [ 2] or applying a scoring system based on thrombus location [ 12, 13].
d, e Activity measurement of covalent and non-covalent functionalized SPIONs after 24 h and 40 days, measured with thrombus-mimicking fibrin-containing agarose plates.
A study on middle cerebral artery (MCA) stroke demonstrated that intravenous thrombolysis had nearly no potential for recanalizing thrombi measuring more than 8 mm in length [ 2].
MCA thrombi measuring more than 8 mm in length were found to have nearly no potential for recanalization with intravenous thrombolysis [ 2].
b Measuring the upper surface.
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