Aliquots of the cell suspension were withdrawn, diluted, and spread onto YPD-agar plates to measure the viable cell number as colony-forming units after a 24-h incubation at 30°C.
Alamar Blue dye reduction method was used to measure the viable cells remaining after drug treatment.
We have shown that with a redox indicator dye, Alamar Blue, we can measure the viable Mtb cell count remaining after treatment with antituberculosis drugs.
Previously, cell population was measured using the OD method, which does not accurately measure the viable cell population.
To assay hybrid fertility, we measured the viable spore yield of Sk/Sp hybrids, Sk/Sk, and Sp/Sp diploids (Smith, 2009).
Algicidal activity was investigated by evaluating the inhibitory rate (IR) of microalgae growth by measuring the fluorescence of viable cells.
We also determined diffusion penetration by measuring the thickness of viable rims of tumor cells within ducts.
The effects of test agents on proliferation were evaluated by directly measuring the number of viable, ie, trypan blue dye (TBD -negative, cells using a hemocyTBD -negative
For measuring the presence of viable cells and the total biomass in the wells, the resazurin staining and crystal violet were used, respectively, using the protocols that have been described earlier [ 18, 19].
Exposure of osteoblasts to the BCM from S. aureus SAMMC-700 significantly reduced cell viability and proliferation as determined by measuring the amount of viable cells and quantification of cellular DNA, respectively.
