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A practical method for measuring the synthesis and decay of mRNA promises new insight into cellular regulation.
Anabolic responses in primary cultured chondrocytes were assessed by measuring the synthesis of proteoglycans in cetylpyridinium-chloride-precipitable fractions of cell-associated and secreted S-labeled macromolecules.
Fibroblast response to HA of various molecular weights was assessed by measuring the synthesis of ECM proteins (elastin and collagen), proliferation, and αSMA expression.
By measuring the synthesis of pre-mRNA directly, we connected the dynamics of the gene with the availability of an upstream transcription factor, the ligand-bound steroid receptor.
Alternatively, assay B was replaced by the radiometric coupled assay method [48], measuring the synthesis of ADP-[C]Glc from [C]Glc-1P and ATP.
The spermidine/spermine N-acetyltransferase (SSAT) activity was determined in the sonicates by measuring the synthesis of [C]acetylspermidine after incubation of the cell extracts with [C]acetyl-coenzyme A and spermidine, as previously described (Matsui et al. 1981).
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This 2H2O technique has been used to measure the synthesis of proteins in muscle, bone, liver, lung and other tissues[6 10].
To measure the synthesis temperature during the reaction, a thermocouple was placed inside the furnace every hour through an injection port without opening the furnace.
Live-cell fluorescent microscopy was used to measure the synthesis and lysosomal turnover of LC3-positive AVs.
We used time-resolved, live-cell microscopy to measure the synthesis and turnover of autophagic vesicles in single cells.
Next, we examined whether the increased CTX-II levels relate to an increased type II collagen synthesis in the rats and therefore measured the synthesis marker PIIANP.
More suggestions(15)
measuring the temperature
measuring the inhibition
measuring the center
measuring the number
measuring the volume
measuring the efficiency
measuring the time
measuring the speed
measuring the space
measuring the phase
measuring the bounce
measuring the output
measuring the value
measuring the scope
measuring the beach
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