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This loss-of-function effect was confirmed by measuring the secretion of endogenous mediators in supernatants of HEK 293T cells (n = 3, p<0.0001; Fig. 4C).
The vaccine- specific cellular immune response in mice was determined using ELISPOT assay measuring the secretion of IFN-γ by splenocytes after stimulation with different peptides pools.
Thus, the clear loss-of-function effect of S183I RIG-I SNP was confirmed by measuring the secretion of IL-8 (n = 3 p<0.0001; Fig. 3D) and RANTES (not illustrated) in the supernatants of stimulated HEK 293T cells.
A proinflammatory response was assessed by measuring the secretion of the pro-inflammatory cytokine interleukin 8 (IL-8) with an ELISA assay (kit from Invitrogen).
Moreover, the activity of specific steroidogenic reactions can be monitored by measuring the secretion of reaction substrate(s) and product(s) from in vitro perfused testes.
Unfortunately, this was not confirmed by biochemically measuring the secretion of VIP from the tumor, because the assay is currently unavailable in mainland China.
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The resulting polychromatic and multiplexed imaging assay enabled us to measure the secretion of mycobacterial effectors inside infected host cells.
Therefore, we transfected the pMAC/PS-scFv into CHO cells, and after 24 hours, we measured the secretion of scFv by dot immunoblotting.
We measured the secretion of IL-8 by DTT-treated Del-N35TCTP, BMOE or BM PEO 3-treated RrTCTP, and BM PEO 3-treated-tRrTCTP DTT-pretreandd-Del-N35TCTP (Figure 5BMOE
We next used ELISA to measure the secretion of cytokine and chemokine proteins from ud- and wd-NHBE cell culture supernatants infected by influenza H1N1 and H5N1 viruses.
Unfortunately, assays for GDF5 were not available to measure the secretion from decidualized cells.
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estimate the secretion
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measuring the center
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measuring the space
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