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Therefore, we used a multi-factor longitudinal design with multiple outcomes, measuring the reading vocabulary knowledge in children with hearing loss from grades 4 and 5, and of children without hearing loss from grade 4, for 3 years with two word tasks: a lexical decision task and a use decision task.
The authors acknowledge that the tool is useful for measuring the reading ability of patients and whilst not an effective measure of comprehensive health literacy, it does provide clinicians with a useful tool to use as a screening instrument.
Capped tube was then kept in a water bath at 45°C for 2 h and the difference between the initial readings is calculated by measuring the reading after 2 h.
Capped tube was then kept in a water bath at 45°C for 2 h and the difference in the initial reading was calculated by measuring the reading after 2 h.
Capped tubes were then kept in a water bath at 45°C for 2 h and the difference with the initial reading was calculated by measuring the reading after 2 h.
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Quality control was performed by measuring the reads per kilobase of TE per million library sequence reads (RPKM) at all TEs, and then combining replicates where the Pearson correlation was >0.6 (Supplementary Figure 1a).
To evaluate read quality, the read length with phred quality ≥ 20 was estimated by measuring the read length after trimming by the qualityTrimmer module of the Euler-SR package [ 33].
This is achieved by measuring the background read density in a large bin surrounding the site ('background read density'; default 3000 bp), and then measuring the read density in a smaller focused bin at the same site ('central read density'; default 300 bp).
As a negative control, we measured the read density under random selections of genomic regions of the same sizes as the CRMs.
To estimate gene expression level, we measured the reads number derived from each contig or isoform using RSEM software package [ 18].
The NAEP Reading Grade 12 assessment (e.g., Perie et al. 2005) measures the reading and comprehension skills of students in grade 12 by asking them to read selected grade-appropriate passages and answer questions based on what they have read.
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