To clarify the mechanism through which ZnO NPs induce apoptosis in LTEP-a-2 cells, we determined the intracellular ROS level by measuring the oxidation of nonfluorescent DCFH-DA to its highly fluorescent derivative DCF.
The level of oxidative stress in L02 cells was determined by measuring the oxidation of DCFH-DA (Beyotime Company, Shanghai, China) as previously described.
The level of intracellular ROS was assessed by measuring the oxidation of 2′,7′-dichlorodihydrofluorescin diacetate as described previously.
Reactive oxygen species (ROS) levels in rat hippocampus were determined by measuring the oxidation of H2DCF-DA as previously described for brain tissue analysis [ 22, 23].
The ROS and NO levels were quantified in monocytes in whole blood by measuring the oxidation of 2,7-dichlorofluorescein diacetate and 4-amino-5-methylamino-2,7-difluorofluorescein 4-amino-5-methylamino-2,7-difluorofluorescein 4-amino-5-methylamino-2,7-difluorofluorescein 4-amino-5-methylamino-2,7-difluorofluorescein 4-amino-5-methylamino-2,7-difluorofluorescein 4-amino-5-methylamino-2,7-difluorofluorescein
By measuring the oxidation of iron in pillow basalts from undersea volcanic eruptions, UC Berkeley scientists have more precisely dated the oxygenation of the deep ocean, inferring from that when oxygen levels in the atmosphere rose to current high levels.
Rats were subjected to graded arterial hypoxia while we measured changes in the oxidation level of cytochrome c oxidase (cytochrome aa3) in the brain by a non-invasive, optical technique.
Therefore we assessed the cellular levels of mitochondrial superoxide by measuring the oxidation-sensitive dye DCFH-DA to examine whether DHODH siRNA-mediated knockdown led to the generation of ROS.
Wu, R. et al. Control of the oxidation level of graphene oxide for high efficiency polymer solar cells.
Normally, the bandgap of RGO increases with the oxidation level.
During this procedure, the oxidation levels of modified HDL were measured (Additional file 1: Figure S1).
