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Uptake of gNO into various media was monitored by measuring the nitrite concentration using the Griess reagent technique.
NO production was indirectly assessed by measuring the nitrite levels in BALF determined by a colorimetric method based on the Griess reaction (Shie et al. 2015).
NO production was tested by measuring the nitrite concentration with the Griess assay according to the method described[15].
NO production in cell culture supernatant was evaluated by measuring the nitrite concentration.
NO production was analyzed by measuring the nitrite formed in the supernatants of cultured RAW 264.7 cells.
The levels of NO in these samples were then evaluated by measuring the nitrite (NO2-) and nitrate (NO3-) content by using a colorimetric assay kit (Biovision research products, Mountain View, CA, USA).
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NO production was assessed by measuring the nitrate and nitrite concentrations according to the commercial kit from Cayman Chemicals, based on the nitrate conversion to nitrite using nitrate reductase followed by the Griess reaction.
We also measured the nitrite and nitrate concentrations produced by erythrocyte suspensions stimulated with the above effectors by means of the Griess reaction method.
Finally, an equal volume of Griess reagent was added into each well to measure the nitrite content.
The level of inflammation was determined by two independent methods: pathological examinations of random bladder biopsies, and testing for bacterial infections using the urine Multistix® 7 test (Bayer A/S Diagnostics Kgs. Lngby, Denmark), which measures the nitrite and leucocyte levels (Robertson and Duff, 1988).
Sixty microliters of two-fold dilution sample were mixed with 60 μl of 10 mM sodium nitroprusside in phosphate buffered saline (PBS) into a 96-wll flat-bottomed plate and incubated under light at room temperature for 150 min. Finally, an equal volume of Griess reagent was added into each well in order to measure the nitrite content.
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