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The ERF properties of neurons were assessed by measuring the neuron's response as a function of stimulus area [4], [28].
The size of neurons was estimated by measuring the neuron area (A) in accordance with the procedure outlined earlier [ 18].
This relationship is often quantified by measuring the neuron's frequency-current curve (F–I curve).
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Shining light on these neurons via optical fibers and then measuring the neurons' responses helped to develop criteria that can identify which responses are generated by the serotonergic neurons.
The trio used multi-electrode arrays cultured with neural cells from rats, stimulated them with drum beats, measured the neuron pulses, then sought relationships between the digitized drum beats and raw data.
The first is physically connecting wires and neurons with microscopic arrays of metallic pins that stick into the brain and electrically stimulate neurons and/or measure the neuron's electric potential when they fire.
We then used computer-controlled presentations of Gabors to measure the neuron's tuning for direction of motion (12 directions), spatial frequency (10 frequencies), and temporal frequency (10 frequencies).
We also measured the neuron density in the part of the septum-related cortex immediately surrounding the C2 column, which was part of the region of interest chosen for manual counting (see red markers in Fig. 2 A ). Neuron density was markedly lower in the septum-related cortex than within a column (71 000 vs. 80 000 per cubic millimeter, cf. Table 1).
MEMS sensor arrays potentially provide a unique advantage for measuring the growth of neurons, if neurons can be isolated from the heterogeneous population.
That initiative, which made its first awards in May (see "Military Funds Brain-Computer Interfaces to Control Feelings"), is broadly focused on developing new technologies for directly measuring the activity of neurons and mapping brain circuits.
The loss of dopaminergic neurons was similarly increased in PGC1α-KO mice (29.1 ± 4.1%) compared to WT mice (10.4 ± 6.3%) when measuring the number of neurons positive for the dopamine transporter (DAT) (Student's t test, p = 0.03).
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