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The kinetic parameters of human PL were determined by measuring the enzyme activity either after the lag phase for native milk fat globules samples or immediately after the addition of the enzyme for homogenized milk samples.
Further, metabolic flux analysis needs to be carried out by measuring the enzyme activities and cofactors to verify this hypothesis.
The substrate specificities of wild-type TsPOx and its mutants were determined by measuring the enzyme activities at pH 6.5 and 30 °C in the McIlvaine buffer containing 100 mM of d-glucose, d-xylose, d-mannose, l-sorbose or d-galactose as the substrate.
The function of recombinant proteins (YxiA1 and YxiA3) was determined by measuring the enzyme activity versus different substrates, like sugar beet linear 1,5-α-l-arabinan, sugar beet red debranched arabinan, xylan from beechwood, wheat flour arabinoxylan, larch arabinogalactan, gum arabic, soluble starch, and 4-nitrophenyl-α-l-arabinofuranoside at the pH 6.0 and 30°C for 30 min.
Total SOD activity in algal thalli was determined by using a Total Superoxide Dismutase Assay kit (Nanjing Jiancheng Bioengineering Institute) based on measuring the enzyme's ability to inhibit the photochemical reduction of tetrazolium blue.
So when measuring the enzyme activities in the isolated mitochondria this represents mainly intact subsarcolemmal mitochondria.
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To measure the enzyme activity 1.5 ml of 0.1 M phosphate buffer (pH-7.5) was added and the mixture was incubated at room temperature for 5 min and then added the enzyme mix.
To accomplish this, we setup a spectrophotomeric assay that measured the enzyme activity of recombinant phosphorylated and nonphosphorylated CN-A/B at high (1.4 µM) and low (40 nM) Ca2+ concentrations.
Representative areas with the most uniform activity of each section were selected to measure the enzyme activity.
We measured the enzyme activity towards both substrates in embryonic axes from imbibing seeds (Fig. 11B and C).
In our standard assay, we measured the enzyme activity in crude cell extracts obtained by ultrasonic treatment.
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