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Because the major source of CO (i.e., more than 80%) in mammals is HO-catalyzed heme catabolism [ 28], and HO-1 is the inducible form of HO responding to various pathological stimuli and stresses, measuring the circulation CO levels may probably a useful way to reflect the expression of HO-1.
Taking into account these context, we investigated the expression and functions of HO-1 in CRC in clinical manifestation in the present study, special attentions being paid to CO production in CRC patients by measuring the circulation carboxyhemoglobin (COHb), which is an easy and economic way to reflect the CO in circulation.
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Furthermore, we measured the circulation kinetics of CD146+ cells before, and then 3 and 12 days after vascular endothelial growth factor (VEGF) antibody blockade by bevacizumab infusion in rectal cancer patients enrolled in a phase I trial.In the peripheral blood of these cancer patients, over 90% of the CD146+ cells were CD45+ hematopoietic cells.
Because bilirubin is also an important product generated by HO-1, we also measured the circulation bilirubin levels in some CRC patients compared to non-tumor patients.
In addition, when we measured the circulation bilirubin that is another important product generated by HO-1, we did not obtain consistent results with CO, no significant increase of circulation bilirubin levels was observed (Additional file 1: Figure S1).
Whole-animal fluorescence images are obtained ventrally at the preassigned time points using IVIS whole-animal imaging system with Living Image software (Xenogen, Caliper Instruments) to measure the circulation serum half-life of the control ssDNA, polymeric DNA, and LbL-ODN-NPs.
In some special cases, the primary node and the secondary node must work together to analyze the data collected from the human body, for example, measuring the blood circulation system.
The aim of the Effects of Saxagliptin on ENdothelial function in patients with type-2 Diabetes (ESENDI -study was to analyzESENDI -studyof saxagliptin on early microvascular changes due to type-2 diabetes by non-invasively measuring the retinal circulation, documenting hemodynamic changes and assessing early vascular remodelling.
These quantities are used by a device that continuously measures the solids circulation rate.
To study in vivo behavior of the nanodrugs, we measured the blood circulation dynamics by recording fluorescence signals of Dox at different time points.
The present results are based on CCN2 measured in the circulation.
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